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Beta2-Glycoprotein-1 IgM EULISA (212596)
品牌：Svar Life Science
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Beta2-Glycoprotein-1 IgM EULISA (212596)
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Beta2-Glycoprotein-1 IgM EULISA

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The EULISA ?2-Glycoprotein-1 IgM is intended for determination of IgM antibodies against ?2-Glycoprotein in human serum. The occurrence of these antibodies is associated with anti-phospholipid syndrome (APS), an autoimmune disorder characterised by thrombosis in both arteries and veins. Antibodies against ?2-Glycoprotein are also believed to be directly involved in the pathogenesis of APS.

Product code
212596
Format
ELISA
Tests
Break apart micro titration strips (12x8) 96 wells
Calculation
Qualitative and quantitative
Antigen
?2-GP-1
Units
U/mL
Calibrators
6
Range
0-100 U/mL
Incubation time
30+30+30 min
Detection system
HRP/450 nm
Availability
CE marked. Not for sale in US
  • Intended use
  • Product Details
  • Kit Components

Intended use

Enzyme immunoassay for the detection of autoantibodies IgM against ?2-Glycoprotein-1.

Background

The anti-phospholipid syndrome (APS) is an autoimmune disorder which can comprise clinical conditions such as venous and arterial thrombosis, thrombocytopenia, myocardial infarction, recurrent spontaneous abortion and neurological complications.

Cardiolipin (CL) is a common, negatively charged, acid phospholipid. Autoantibodies associated with APS are directed not only against CL and similar phospholipides but also against phospholipid/protein complexes. ?2-glycoprotein 1 (?2-GP-1; = apolipoprotein H) has been identified as a natural and essential co-antigen for CL-autoantibodies. The occurrence of CL/?2-GP-1 autoantibodies is associated with a tendency towards thrombosis.

Technical information

The EULISA ?2-Glycoprotein-1 IgM is intended for the quantitative or qualitative determination of IgM antibodies in human serum, directed against ?2-GP-1. The immobilised antigen is a highly purified preparation of human ?2-GP-1.

The test is fast (incubation time 30-30-30 minutes) and flexible (break apart wells, ready-to-use reagents). Six calibrators allow quantitative measurements; a negative and a positive control are used to check assay performance.

Kit components and storage of reagents

Contents of the kit

  1. 1 microwell plate, coated with ?2-GP-1 and hermetically packed in a foil laminate pouch together with a desiccant bag.
  1. Sample buffer, 100 mL, ready-to-use, orange coloured.
  1. Wash buffer, 100 mL, 10 x-concentrate, blue coloured.
  1. 6 calibrators, 2.0 mL each, 0, 3.0, 8.0, 18, 45 and 100 U ?2-GP-1-Ab (IgM)/ mL, ready-to-use, gradually blue coloured.
  1. Negative and positive control, 2.0 mL each, ready-to-use, green and red coloured, respectively.
  1. Anti-human IgM HRP conjugate, 14 mL, ready-to-use, red coloured.
  1. Substrate solution, 14 mL, ready-to-use, colourless.
  1. Stop solution (0.5 M H2SO4), 14 mL, colourless, ready-to-use.
  1. Directions for use
  1. Lot-specific certificate of analysis

All kit components are stored at 2 - 8°C.

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